The study on the kinetics of the enzyme showed that the enzymatic reaction with lactic acetate as the substrate accorded with the michaelis - menten equation . it michaelis constant km was 5 . 77 , and the maximum velocity was 0 . 45 u mol / l min 酶动力学研究表明：该酯酶在以乳酸乙酯为底物的酶促反应符合米氏方程，米氏常数为km ： 5 . 77 ，最大反应速度vmax为： 0 . 45 mol l ? min 。
2.
A novel catalytic property of hemin is described . in ammonium buffer medium of ph 8 . 08 . 9 , hemin has an ability to catalyze the decoloration of bromopyro - gallol red . oxygen dissolved in solution plays a role as an oxidant in the reaction . with sodium dodecylbenzenesulfonate and ethanol as effect - enhancing agents , the catalytic system obeys the michaelis - menten equation of enzymatic catalysis . the michaelis constant , catalytic constant and specificity constant of the system are estimated by lineweaver - burk plot to be 3 . 710 在ph 8 . 08 . 9氨性缓冲介质中， hemin催化溶解氧氧化溴邻苯三酚红的褪色反应。在sdbs和30 %乙醇的增效作用下，催化体系服从michaelis - menten方程。用lineweaver - burk作图法求得米氏常数催化常数和专一性常数分别为3 . 710
